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. 2014 May 13;9(5):e97123. doi: 10.1371/journal.pone.0097123

Figure 8. Effect of muscone on BMSC migration capacity.

Figure 8

A: Quantification of the BMSC migration capacity using scrape-healing assay and transfilter assay. The number of migrating BMSCs was counted in each field. BMSCs exposed to CXCR4 and CXCR7 neutralizing antibodies were used for the both assay, and the promigratory effect of muscone on BMSC was abrogated in different degree. *P<0.05 compared with the corresponding group without the treatment of CXCR4 or CXCR7 neutralizing antibody. B: Enhancement of BMSC migration in vitro. Representative images of BrdU-positive cells in the BMSC and combined groups. The number of BrdU-positive cells was further counted in each field. Scale bar corresponds to 30 µm. C and D. Expression of CXCR4 and CXCR7 in BMSCs with or without muscone treatment detected using qPCR and western blot, respectively. Similar results were obtained in at least three independent experiments. Results are expressed as mean ± SEM. A t-test was used to compare the various groups, and P<0.05 was considered statistically significant. *P<0.05 compared with the normal BMSC group without any muscone treatment.