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. Author manuscript; available in PMC: 2014 May 13.
Published in final edited form as: Drug Dev Res. 2014 Mar 11;75(3):172–188. doi: 10.1002/ddr.21169

Fig. 1.

Fig. 1

A carbohydrate microarray analysis of oligomannosyl antigens for expression of the 2G12-like and GNA-like glyco-epitopes. The mannose-cluster-containing microarrays were stained with 2G12 (5 μg/ml) and a biotinylated GNA (1.0 μg/ml), respectively. The former was revealed by Alexa647-tagged Goat anti-human IgG-Fc-specific antibodies at 2 μg/ml; then developed with Streptavidin-Cy5 conjugate at 2 μg/ml. (A) Microarray images stained with either GNA or 2G12. (B) Cluster configuration of the three Man9-conjugates. Microarray detections are shown as the mean fluorescent intensities (MFIs) of each microspot as captured by the ScanArray 5000A for the arrays stained with GNA (C) and 2G12 (D), respectively. Results were compared using overlay plots of the MFIs of staining signal (circles) versus those of local backgrounds surrounding the antigen microarrays (crosses) (C and D). [Color figure can be viewed in the online issue which is available at wileyonlinelibrary.com]