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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2013 Nov 27;9(3):277–284. doi: 10.1007/s11481-013-9516-y

Fig 3. Effect of MAPK and phosphatase inhibitors on Li’s effect on LPS-induced Ccl2 mRNA expression in human macrophages.

Fig 3

(A). Cells were treated with vehicle or U01206 (10 μM) for 30 min followed by LPS (2 ng/ml) or LiCl (30 mM) for 12 hours. RNA was extracted and Ccl2 mRNA expression determined by real time Q-RT-PCR. (B). THP-1X-blue macrophages were pretreated with vehicle, okadaic acid (OA, 100 nM) or sodium orthovanadate (Na3VO4) for 30 min followed by treatment with NaCl (30 mM), or LiCl (30 mM) for 12 hours. These treatments were followed without or with LPS (2 ng/ml) for 30/60 min (for pERK) or 12 hours (for Ccl2 mRNA expression). PhosphoERK levels and Ccl2 mRNA expression were determined as described in Fig 1 and 2. N=3. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.