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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Thorac Cardiovasc Surg. 2013 Nov 13;147(6):1939–1945. doi: 10.1016/j.jtcvs.2013.09.054

Fig. 2. Aprotinin prevents serum deprivation-induced apoptosis in cortical neurons.

Fig. 2

(a) Percentage of trypan blue+ cells at 24hrs after serum deprivation. Ten percent cell death in Control supports a technical consistency in our study.35 Aprotinin at 50, 100 and 200KIU/ml decreased trypan blue+ cell percentage from 75% to 60%, 37% and 34%, respectively. (b) Cell viability using MTT assay after 24hr SD. Aprotinin at 100 and 200 KIU/ml significantly increased the viability compared with No Aprotinin (p < 0.05). There was no significant difference between No Aprotinin and 50KIU/ml Aprotinin. (c-g) Images in Hoechst 33258 staining at 24hrs after Sham wash (c) and after SD with Aprotinin at the concentration of 0 (d), 50 (e), 100 (f), and 250KIU/ml (g). *p < 0.05, **p < 0.01, ***p < 0.001 vs. Aprotinin 0KIU/ml by ANOVA with Bonferroni comparisons. Data are shown as mean ± SEM (n = 8).