Figure 5.

PAK inactivation reduced Aβ and tau depositions in 12- and 20-month-old heterozygous 3xTg-AD mice. PAK inactivation did not alter concentrations of sAβ40 (A) and sAβ42 (B) in soluble fractions at any age, but decreased iAβ40 (C) and iAβ42 (D) levels in insoluble fractions from 12-month-old 3xTg-AD mice. Whereas the inactivation of PAK induced a decrease of the insoluble/soluble tau ratio in 12-month-old mice (E), it did not induced any effect on the soluble phospho-tau/total tau ratio in mice whatever their age (F). Finally, no effect was observed on phospho-tau levels in the insoluble fraction (G). Values are expressed as mean ± SEM. Statistical comparisons were performed using an unpaired t test. ROD indicates relative optical density. Comparison between genotype: *p < 0.05, **p < 0.01; comparison between age: ^#p < 0.05, ^##p < 0.01, ^###p < 0.001.