Figure 5.

Phenotypic characterization of the immune system. A, Dot plots showing frequency of B220⁺ B-cell precursors in bone marrow including: pre-pro B cells (CD43⁺CD19⁻), pro-B cells (CD43⁺CD19⁺), pre-B cells (CD43⁻CD19⁺CD2⁻B220^int), and mature B cells (CD43⁻CD19⁺CD2⁺B220^high). B, Representative histograms showing the percentage of cells expressing the activation marker LFA-1 on CD4⁺ and CD8⁺ cells isolated from lymph nodes (summarized in bar graphs). C, Representative dot plots showing frequency of T-cell precursors in thymus. CD4⁻CD8⁻ double-negative (DN) cells were subdivided into developmental stages DN1 (CD44⁺CD25⁻), DN2 (CD44⁺CD25⁺), and DN3 (CD44⁻CD25⁺). CD4⁺ and CD8⁺ single-positive populations are also shown, along with percentage of cells expressing the T-cell coreceptor CD3 (histogram). D, Frequency of granulocytic CD11b^highLy6g⁺SSC^high and monocytic CD11b⁺Ly6g⁻SSC^low myeloid cells in the spleen. E, T-helper lineage-specific intracellular cytokine production among lymphocytes stimulated with anti-CD3 and anti-CD28 for 4–5 h. Representative dot plots show IL-17 (Th17) and IFNγ (Th1); IL-4 (Th2) and IL-10 (T_reg) are not depicted. In all panels, values are the mean (n = 3) ± SD. *p < 0.05; **p < 0.005; ***p < 0.0005.