Figure 5.

The Opt allele rescues enhanced hippocampal potentiation in M146V mice. A, B, Basal synaptic transmission in hippocampal region CA1 as measured by slope of the input-output curve, the ratio of fEPSP slope to PFV amplitude at each applied voltage, and the maximum evoked fEPSP slope for slices from 5-week-old mice. Unpaired two-tailed t test, n ≥ 6 mice each, no significant differences observed. C, Hippocampal LTP resulting from a single 100 Hz, 1 s tetanus applied at t = 0 in slices from 5-week-old mice. The average of the baseline fEPSPs (black) and those recorded during the last 20 min (red) is presented. Comparisons with WT were made over the first 10 min after tetani and over the last 20 min of the recording. Repeated-measures ANOVA, n ≥ 5 mice each, p < 0.005. D, E, Basal synaptic transmission in hippocampal region CA1 as measured by slope of the input-output curve, the ratio of fEPSP slope to PFV amplitude at each applied voltage, and the maximum evoked fEPSP slope for slices from 3-month-old mice. Unpaired two-tailed t test, n ≥ 6 mice each, no significant differences observed. F, Hippocampal LTP resulting from a single 100 Hz, 1 s tetanus applied at t = 0 in slices from 3-month-old mice. The average of the baseline fEPSPs (black) and those recorded during the last 20 min (red) is presented. Comparisons with WT were made over the first 10 min after tetani and over the last 20 min of the recording. Repeated-measures ANOVA, n = 5 mice each, *p < 0.05, **p < 0.005. Error bars show mean and SEM.