Figure 8.

The Opt allele attenuates cortical AD-like pathology in 3xTg mice. A, Western blot analyses of cortical lysates from 3- to 18-month-old mice using antibodies specific for InsP₃R1 (normalized to WT), hAPP (6E10), htau (HT7), and tubulin as loading control. Representative blots from experiments conducted on 18-month-old mice. Unpaired two-tailed t test, n ≥ 3 mice each per age, p ≤ 0.005. B, ELISA of cortical homogenates for Aβ₄₀ and Aβ₄₂ in mice ranging in age from 6 to 18 months. Aβ content in homogenates from WT and Opt animals were always below the ELISA detection limit. Unpaired two-tailed t test, n ≥ 4 mice each per age, *p ≤ 0.05, **p ≤ 0.005. C, Reverse transcriptase RT-PCR for htau expression using cortical RNA isolated from 6-month-old mice. Unpaired two-tailed t test, n = 3 mice each, p = 0.06. Error bars show mean and SEM.