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. 2014 May 14;34(20):6924–6937. doi: 10.1523/JNEUROSCI.0694-14.2014

Figure 5.

Figure 5.

Sucrose density gradients of wt and mutant DVMAT expressed in octopaminergic neurons. A, Representative Western blots show sucrose gradients of homogenates from fly heads expressing DVMAT-wt, DVMAT-Δ3, or DVMAT-Y600A, as indicated, using the Tdc2-Gal4 driver. B, Quantification of immunoblots (n = 3–5 per genotype) for DVMAT-wt, DVMAT-Δ3, and DVMAT-Y600A, as indicated. Compared with that of DVMAT-wt, HA immunoreactivity representing DVMAT-Δ3 and DVMAT-Y600A is shifted to fractions that contain LDCVs. Shaded bars show the position of markers for SVs and LDCVs. C, D, Total immunoreactivity representing DVMAT differed between genotypes for SV-containing fractions (C, ANOVA, p = 0.035, Dunnett's multiple-comparison test vs wt, *p < 0.05) and LDCV-containing fractions (D, ANOVA, p = 0.03, Dunnett's multiple-comparison test vs wt, *p < 0.05, as indicated).