Fig. 4.

miR-21 inhibition affect neurite outgrowth in DRG cells. a, b Immunofluorescence staining. a Control, untransfected DRG cells, b DRG cells transfected with an EGFP encoding plasmid (control EGFP) and with the plasmid encoding the miR-21 sponge. Blue Hoechst staining; red ß-III-tubulin; green GFP. Scale bar 15.5 μm. c The histogram shows the mean length of neurites express in μm. Data are represented as means ± s.e.m. of triplicate determinations of ten random fields in four independent experiments. A total of 169 cells were counted for the not transfected control, a total of 133 cells were counted for EGFP control, and a total of 170 cells were counted for the miR-21 sponge. P values were calculated by one-way ANOVA followed by Tukey’s multiple comparison test; ***P value ≤0.01