Table 1.
MicroRNAs modulated by Ngf appear to be involved in cell signaling
| KEGG Pathway | p value | #genes | #miRNAs |
|---|---|---|---|
| A 1 h Ngf | |||
| TGF-beta signaling pathway | <0.01 | 15 | 6 |
| Neurotrophin signaling pathway | <0.01 | 17 | 6 |
| Circadian rhythm | 0.02 | 5 | 5 |
| ECM-receptor interaction | 0.02 | 6 | 3 |
| SNARE interactions in vesicular transport | 0.02 | 6 | 6 |
| JAK-STAT signaling pathway | 0.02 | 18 | 8 |
| B 3 h Ngf | |||
| Long-term depression | 0.02 | 9 | 5 |
| MAPK signaling pathway | 0.02 | 24 | 5 |
| Focal adhesion | 0.04 | 19 | 5 |
| TGF-beta signaling pathway | 0.04 | 11 | 4 |
| C 6 h Ngf | |||
| ECM-receptor interaction | <0.01 | 9 | 4 |
| Focal adhesion | <0.01 | 24 | 7 |
| Axon guidance | 0.02 | 14 | 6 |
| TGF-beta signaling pathway | 0.04 | 10 | 6 |
| Adherens junction | 0.04 | 9 | 6 |
| D 24 h Ngf | |||
| Axon guidance | <0.01 | 30 | 8 |
| MAPK signaling pathway | 0.04 | 43 | 7 |
| Long-term depression | 0.04 | 13 | 6 |
| E 240 h Ngf | |||
| TGF-beta signaling pathway | <0.01 | 50 | 39 |
| Wnt signaling pathway | <0.01 | 89 | 41 |
| Axon guidance | <0.01 | 74 | 41 |
| Neurotrophin signaling pathway | <0.01 | 72 | 44 |
| Focal adhesion | <0.01 | 103 | 40 |
| Long-term potentiation | <0.01 | 42 | 40 |
| ErbB signaling pathway | <0.01 | 48 | 39 |
| MAPK signaling pathway | 0.02 | 125 | 43 |
Predicted targets of all microRNAs modulated by Ngf at each time point—from 1 to 240 h—were retrieved and mapped into the KEGG Pathway database through DIANA miR-Path software; only pathways with p value ≤ 0.05 were retained. Pathways relevant to nervous system and neurons are shown; the number of Ngf-modulated miRs targeting a given pathway and the numbers of genes that are targeted in that pathway are also indicated