Table 2.
Characteristics distinguishing A. glycyphyllos symbionts from other Mesorhizobium species
| Characteristics |
Astragalus glycyphyllos
Nodule isolates |
M. tianshanense USDA3592 | M. chacoense USDA4963 | M. mediterraneum USDA3392 | M. plurifarium USDA3707 | M. ciceri USDA3383 | M.huakuii USDA4779 | M. amorphae ICMP15022 |
M. loti
USDA3471 |
|---|---|---|---|---|---|---|---|---|---|
| n a = 28 | n = 1 | n = 1 | n = 1 | n = 1 | n = 1 | n = 1 | n = 1 | n = 1 | |
| Tolerance to pH | |||||||||
| 4 | −b | − | − | − | +b | − | − | − | − |
| 5 | − | − | + | − | + | + | + | + | + |
| Tolerance to NaCl (%) | |||||||||
| 2 | + (9)c | − | + | + | + | + | − | + | − |
| Enzyme activity | |||||||||
| Glucohydrolase-d-glucoside | + (12) | − | − | + | − | − | + | − | − |
| Phosphatase | + (8) | + | + | + | + | + | + | + | + |
| Nitrate reductase | + (15) | − | − | − | − | − | − | − | − |
| Others | |||||||||
| IAA | + (6) | + | − | − | − | − | + | − | − |
| Calcofluor, UV | + (20) | + | + | + | + | − | + | + | + |
| Nitrogen source | |||||||||
| l-Histidine | + (23) | + | − | + | − | + | + | − | + |
| l-Serine | + (9) | − | + | − | − | − | + | − | + |
| NH4Cl | + (6) | + | + | + | + | + | + | + | + |
| dl-Isoleucine | − | − | − | − | − | − | + | − | + |
| l-Hydroxyproline | + (13) | + | + | + | + | + | + | + | + |
| NaNO3 | + (12) | − | + | − | + | − | + | + | + |
| Carbon source | |||||||||
| l-Arginine | + | + | + | + | + | − | + | + | + |
| l-Lysine | + (23) | − | + | + | + | − | − | + | + |
| Salicin | + | − | + | − | + | − | + | + | − |
| Sodium citrate | + | − | + | − | + | − | + | − | − |
| Starch | + (5) | − | − | − | − | + | − | − | + |
| l-Alanine | + (7) | − | + | − | − | − | + | + | − |
| l-Asparagine | + (20) | − | − | − | + | + | + | + | + |
| Sodium tartrate | − | − | + | − | + | − | − | + | − |
| l-Tyrosine | + (24) | − | − | − | + | − | − | − | + |
| l-Glutamine | + (21) | − | − | − | − | − | − | − | − |
| l-Histidine | + (7) | − | + | − | − | + | − | + | + |
| Tolerance to dyes (%) | |||||||||
| Crystal violet | |||||||||
| 0.0065 | + (7) | + | + | + | + | + | + | + | + |
| 0.013 | − | − | − | + | − | + | + | + | + |
| Nigrosine | |||||||||
| 0.5 | + (21) | − | − | − | + | − | − | + | − |
| Safranine | |||||||||
| 0.05 | + (20) | − | − | + | + | + | + | + | + |
| Methyl green | |||||||||
| 0.0065 | − | − | + | + | + | + | + | + | + |
| 0.013 | − | − | − | − | + | − | + | − | − |
| Tolerance to antibiotics (g ml−1) | |||||||||
| Neomycin | |||||||||
| 10 | + (12) | − | − | − | − | − | + | + | − |
| Streptomycin | |||||||||
| 10 | + (20) | − | − | + | − | + | − | + | − |
| Rifampicin | |||||||||
| 2.5 | + (11) | + | + | + | + | − | + | + | + |
| 10 | + (6) | − | + | − | − | − | + | + | − |
| Chloramphenicol | |||||||||
| 50 | + (20) | − | + | − | − | − | − | + | − |
| Tetracycline | |||||||||
| 10 | + (15) | − | − | − | − | − | − | − | − |
| Ampicillin | |||||||||
| 10 | + (17) | − | − | − | + | − | − | + | − |
aNumber of studied strains
b+, − Strains were positive, negative, respectively
cNumerical value in parentheses is the number of strains with positive reaction