Table 3.
RFLP analysis of PCR-amplified 16S rDNA of A. glycyphyllos isolates and reference mesorhizobial strains used in this study
| Strains | Restriction pattern typesa of amplified 16S rDNAs digested with: | 16S rDNA–RFLP genotypesb | 16S rDNA–RFLP patterns groupsc | ||||||
|---|---|---|---|---|---|---|---|---|---|
| AluI | MboI | RsaI | Hin6I | TaqI | MspI | HinfI | |||
| AG1 | A | A | A | A | A | B | A | AAAAABA | I |
| AG2 | A | A | A | C | A | B | A | AAACABA | II |
| AG3 | A | A | A | C | A | B | A | AAACABA | II |
| AG4 | A | A | A | A | C | B | A | AAAACBA | III |
| AG5 | A | A | A | A | A | B | A | AAAAABA | I |
| AG6 | A | A | A | A | A | B | A | AAAAABA | I |
| AG7 | A | A | A | A | A | B | A | AAAAABA | I |
| AG8 | A | A | A | C | A | B | A | AAACABA | I |
| AG9 | A | A | A | C | A | B | A | AAACABA | I |
| AG10 | A | A | A | C | B | B | A | AAACBBA | IV |
| AG11 | A | A | A | A | B | B | A | AAAABBA | V |
| AG12 | A | A | B | A | A | A | E | AABAAAE | VI |
| AG13 | A | A | A | B | A | D | B | AAABADB | VII |
| AG14 | A | A | A | B | A | D | B | AAABADB | VII |
| AG15 | A | A | A | B | A | D | B | AAABADB | VII |
| AG16 | A | A | A | A | C | A | B | AAAACAB | VIII |
| AG17 | A | A | A | A | A | A | B | AAAAAAB | IX |
| AG18 | A | A | B | A | A | A | B | AABAAAB | X |
| AG19 | A | A | B | C | A | A | B | AABCAAB | XI |
| AG20 | A | A | A | A | A | C | A | AAAAACA | XII |
| AG21 | A | A | B | A | A | C | A | AABAACA | XIII |
| AG22 | A | A | B | A | A | A | B | AABAAAB | X |
| AG24 | A | A | B | A | A | C | A | AABAACA | XIII |
| AG25 | A | A | B | A | B | C | A | AABABCA | XIV |
| AG26 | A | A | B | B | A | D | B | AABBADB | XV |
| AG27 | A | A | B | A | B | C | A | AABABCA | XIV |
| AG28 | A | A | B | A | A | C | A | AABAACA | XIII |
| AG29 | A | A | B | A | A | C | A | AABAACA | XIII |
| Mesorhizobium albiziae CCBAU61158 | A | B | C | B | D | E | F | ABCBDEF | XVI |
| Mesorhizobium amorphae ICMP15022 | A | A | A | B | A | A | B | AAABAAB | XVII |
| Mesorhizobium caraganae CCBAU11299 | A | A | A | A | A | C | C | AAAAACC | XVIII |
| Mesorhizobium ciceri USDA3383 | A | A | A | A | A | D | C | AAAAADC | XIX |
| Mesorhizobium chacoense USDA4963 | A | A | A | D | A | F | B | AAADAFB | XX |
| Mesorhizobium gobiense CCBAU83330 | A | A | D | A | A | G | H | AADAAGH | XXI |
| Mesorhizobium huakuii USDA4779 | A | A | A | B | A | A | B | AAABAAB | XVII |
| Mesorhizobium plurifarium USDA3707 | A | A | A | A | E | A | D | AAAAEAD | XXII |
| Mesorhizobium loti MAFF303099 | A | A | A | B | A | A | D | AAABAAD | XXIII |
| Mesorhizobium septentrionale SDW018 | A | A | A | A | A | A | B | AAAAAAB | IX |
| Mesorhizobium shangrilense CCBAU65327 | A | A | A | A | A | A | F | AAAAAAF | XXIV |
| Mesorhizobium tianshanense USDA3592 | A | A | A | A | A | C | A | AAAAACA | XII |
| Mesorhizobium temperatum LMG23931 | A | A | A | B | A | C | C | AAABACC | XXV |
aLetters (A–G) refer to 16S rDNA–RFLP pattern types of studied rhizobia detected with each used restriction enzyme
bCombinations of letters (A–G) refer to 16S rDNA genotypes of studied rhizobia detected by the combined RFLP analyses of 16S rDNA digested with MspI, MboI, Hin6I, HinfI, TaqI, RsaI, and AluI endonucleases
cRoman numerals refer to 16S rDNA–RFLP pattern groups of studied rhizobia identified by the combined analyses of 16S rDNA restriction profiles obtained with seven endonucleases used