Table 1.

Percentage of viruses identified in DNA viromes by BLAST comparison to the NCBI viral Refseq genome database (bit-score = 50, GAAS normalized).

Virus type	CFM	MECH
		NLN	LN2	NLN*	LN2*
dsDNA viruses, no RNA stage	11.3	10.9	6.6	78.9	71.9
Bacteriophage	(98.6%)	(93.5%)	(93.5%)	(99%)	(99%)
Eukaryotic	(1.4%)	(6.5%)	(6.5%)	(1%)	(1%)
Reverse transcribing viruses	0	1.8	4.9	0.4	1.0
Satellites	3.3	4.7	6.1	0.9	0.1
ssDNA viruses	84.9	82.1	82.3	17.5	24.6
ssRNA viruses	0	<0.1	<0.1	<0.1	<0.1
Unclassified archaeal viruses	0	<0.1	0	0	<0.1
Unclassified phages	0.4	0.4	0.1	2.2	2.3
Unclassified viruses	<0.1	0.1	<0.1	<0.1	0.1
Virophage	<0.1	0	0	<0.1	<0.1

The viral metagenomic data sets generated by the previously published chloroform treatment method (CFM) and our novel mechanical disruption method (MECH). The latter method was tested with (LN2) and without (NLN) liquid nitrogen tissue preservation. Data sets with an asterisk (^*) were amplified using RP-SISPA prior to sequencing and were analyzed after removal of contaminating ssDNA phiX sequences (spiked during Illumina sequencing). All other data sets were amplified using RepliG® technology. Percent of dsDNA viruses as phage or eukaryotic viruses are highlighted in brackets.