Figure 1.

Phosphatases affecting the RAS-PI3K pathway. (A) The results depicted in this figure are a subset of the results reported by Sacco et al. (2012a). The squares represents the main proteins that are involved in the PI3K and RTK/RAS signaling cascades. Green squares are the phosphorylated proteins that have been used as readouts in the screening. The phosphatase whose down regulation was shown to affect the readouts are represented as circles and are linked to readouts with red or green edges depending on whether their down-regulation negatively or positively affected the readouts. The intensity of the edge red or green color represents the trust in the reported interaction (number of concordant siRNA). The color of the phosphatase nodes is mapped to the phosphatase family according to the figure legend. (B) As previously described by Sacco et al., after phosphatase down-regulation the activation level of ERK and rpS6 was analyzed by automated immunofluorescence microscopy. Here, we report the ERK and rpS6 phosphorylation level after the down-regulation of some phosphatases hits.