Figure 6.

(A) Schematic of experimental design. Rats were pre-trained on the reward cue-reward pellet association followed by 4 Discriminative Conditioning sessions in which the reward cue (+reward pellet), fear cue (+footshock), fear+safety cues (no footshock) and safety cue (no footshock) were presented. During Extinction Acquisition all rats received unreinforced presentations of the fear and reward cues. Extinction Recall occurred the next day and consisted of unreinforced presentations of all cues. Data are presented below for the sessions indicated by white boxes. (B, left) Both groups demonstrated significant within session extinction of reward-seeking behavior to the reward cue. Percent time in port to each reward cue presentation is shown. Reward seeking to the last cue presentation was significantly lower compared to the first cue presentation for both groups. PolyI:C-treated offspring displayed significantly lower percent time in port during trials 12, 16, 17, and 24 (^*p < 0.05). (B, right and C) During Extinction Recall no differences in reward seeking were detected between groups during any cue. Both groups showed a significant reduction in reward seeking to the reward cue compared to the beginning of Extinction Acquisition. Panel (C) shows reward seeking during the first presentation of each cue type on the Extinction Recall test. (D) Reward seeking during the 20 s pre-reward cue and 20 s post-reward cue was the same for both groups. (E) The total percent spent in port during the first 180 s of Extinction Recall was not different between groups.