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. 2014 May 7;5:202. doi: 10.3389/fmicb.2014.00202

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Copyright © 2014 Parker, Lee, Geszvain, Davis, Gruffaz, Meyer, Torpey and Tebo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Clusters of putative genes likely to be involved in PVD synthesis in P. putida GB-1. Identification is based on in silico comparison to known PVD-related genes, as described in the text. Genes are annotated according to Ravel and Cornelis (2003). Color schemes are based on Ravel and Cornelis (2003). Numbers on top are locus tags for P. putida GB-1 (PputGB1 numbers). Length is not to scale. (B) The 8 predicted adenylation domains found in the putative NRPSs (PputGB1_4083-4086) in P. putida GB-1, and the resulting peptides of each adenylation domain predicted using NRPSpredictor and cluster analysis in comparison with the reported peptide sequence of PVD_CFML90-51. The NRPS genes are shown in green with the adenylation domains boxed in blue.