Table 2.
Properties of various Pseudomonas sp. strains examined.
| Strain | Pseudomonas species or group (gp), from 16S rRNAa | Forms MnO2 | CAS reactionb (PVD reaction)b | Sidero-typec | 59Fe-PVD uptake (% of homologous uptake)d | Isolated frome (references in footnote) |
|---|---|---|---|---|---|---|
| CFML 90-45 | putida | No | CAS+ (PVD+) | 1 | CFML 90-51 (>90%) | Clinical specimen |
| CFML 90-48 | putida | No | CAS+ (PVD+) | 1 | CFML 90-51 (>90%) | Clinical specimen |
| CFML 90-49 | putida | No | CAS+ (PVD+) | 1 | CFML 90-51 (>90%) | Clinical specimen |
| CFML 90-50 | putida | No | CAS+ (PVD+) | 1 | CFML 90-51 (>90%) | Clinical specimen |
| CFML 90-51 | putida | No | CAS+ (PVD+) | 1 | CFML 90-51 (100%) | Clinical specimen |
| GB-1 | putida | Yes | CAS+ (PVD+) | 1 | CFML 90-51 (95%) | Freshwater sediment |
| MnB1 | putida | Yes | CAS+ (PVD+) | 1 | CFML 90-51 (104%) | Freshwater pipe |
| KT2440 | putida | Yes, at low O2 | CAS+ (PVD+) | 2 | F317 (91%) | Soil, toluate deg |
| ATCC 55241 | fluorescens biotype II BNL-WVC | No | CAS+ (PVD+) | 3 | No match to known PVD | Radiowaste leachate |
| ISO6 | fluorescens-syringae gp. | Yes, at low O2 | CAS+ (PVD+) | 4 | Metallogenium particles | |
| PCP1 | fluorescens-syringae gp. | Yes | CAS+ (PVD+) | 4 | D47, SB8.3 (~50% each) | Sediment, mine drainage |
| MG1 | fluorescens-syringae gp. | Yes | CAS+ (PVD+) | NTf | Metallogenium particles | |
| ISO1 | fluorescens-syringae gp. | Yes | CAS+ (PVD−) | NAg | Metallogenium particles | |
| GP11 | stutzeri gp. | Yes | CAS− (PVD−) | NA | Pulpmill effluent | |
| SI85-2B | marincola | Yes | CASNT (PVD−) | NA | Marine bay, suboxic | |
| PCP2 | mendocina-resinovorans gp. | Yes | CAS− (PVD−) | NA | Sediment, mine effluent |
The chrom azurol S (CAS) method, which depends on the ability of siderophores to displace Fe from its CAS complex, is a general assay for siderophores (Schwyn and Neilands, 1987). The presence of pyoverdine-group siderophores (PVD) was detected from UV-vis absorption and fluorescence spectra (Parker et al., 2007). A strain was scored positive (+) if a CAS reaction or PVD was detected.
Based on the isolectric focusing pattern of each strain's fluorescent PVD and confirmed by each strain's uptake of 59Fe-PVD from 34 standard strains, using methods in Fuchs et al. (2001), but with assigning of our own siderotype numbers.
FePVD standard that was taken up in greatest amount. (% uptake compared to that of the homologous standard strain).
Clinical specimen or from associated medical environment, Collection de la Faculté de Médecine de Lille, France (Meyer et al., 2007); freshwater pipe encrusted with MnO2, Germany (Schweisfurth, 1973); freshwater sediment, Green Bay of Lake Michigan, USA (Francis and Tebo, 2001); laboratory variant selected by Brandy Toner in the Garrison Sposito laboratory, University of California Berkeley, USA; marine fjord, oxic-anoxic interface, Saanich Inlet, Vancouver Island, BC, Canada (Emerson et al., 1982; Francis and Tebo, 2001; Romanenko et al., 2008); Metallogenium particles from Horsetooth Reservoir, Fort Collins, CO, USA (Francis and Tebo, 2001); pulpmill effluent, Grande Prairie, AB, Canada (Francis and Tebo, 2001); radiowaste leachate, low-level radioactive waste leachate, Brookhaven Natl. Lab., USA (Francis and Dodge, 1998); sediment, mine drainage, Pinal Creek, Globe, AZ, USA, downstream from a Cu mine (Fuller and Harvey, 2000; Francis and Tebo, 2001); soil, toluate deg, soil enrichment for degradation of toluate, Osaka, Japan (Nakazawa, 2002; Regenhardt et al., 2002).
NT, not tested. Strain MG-1 did not grow at the standard conditions used for IEF analysis and 59Fe PVD uptake.
NA, not applicable because that organism does not make PVD.