Table 3.
Mass spectrometric (MS/MS) analysis of siderophores from several siderotype n° 1 strains of Pseudomonas putida.
P. putida strain | Pyoverdine m/z (isotope cluster area, %)a | Azotobactin m/z (isotope cluster area, %)a |
---|---|---|
CFML 90-51 | 1251.57 (53.6%) | 1161.52 (26.8%) |
GB-1 | 1250.57 (30.7%) | 1161.53 (53.9%) |
MnB1 | 1250.34 | 1161.34 |
The mass spectrometric (MS) isotope cluster area is the sum of the peak areas of all isotopes associated with each monoisotopic m/z measurement, expressed as a percentage of all PVD-type molecules made by that strain. The peptide sequence of each siderophore was determined by analysis of the second sequential set of MS/MS peaks and was, in all cases: chromophore-Asp-Lys-OHAsp-Ser-Gly-aThr-Lys-cOHOrn. The small differences in weights and slight variations in malonate substitution are described in the text.