Figure 2.

Effect of chamomile on cell viability and ROS generation with H₂O₂ in RAW 264.7 cells. (A) Cell viability assay with MTT on RAW 264.7 cells treated with chamomile at concentrations 0–40 μg/mL for 16 h and further challenged with H₂O₂ at 50, 100, and 200 μM for 6 h; (B) ROS assay with DCF-DA on RAW 264.7 cells treated with chamomile at concentrations 0–40 μg/mL for 16 h and further challenged with H₂O₂ at 50, 100, and 200 μM for 6 h; (C) photographs of RAW 264.7 macrophages (subpanel a–d) showing cells after treatment with 50 μM H₂O₂ for 6 h, 20 μg/mL chamomile for 16 h alone and chamomile with H₂O₂. The bars represent mean ± SD of at least three independent experiments each performed in triplicate, *p <0.05 and **p <0.001 represent significant differences as compared with the chamomile and H₂O₂ treated group. ^¶p <0.05 and ^#p <0.001 represent significant differences as compared with the H₂O₂ model group. The details are described in the Materials and methods section.