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. Author manuscript; available in PMC: 2014 May 14.
Published in final edited form as: Phytother Res. 2012 Apr 18;27(1):118–125. doi: 10.1002/ptr.4701

Figure 4.

Figure 4

Effect of chamomile on Nrf2/Keap1 expression in RAW 264.7 macrophages. (A) Western blot for cytosolic Nrf2 and Keap1 protein expression, and (B) nuclear expression of Nrf2 in RAW 264.7 macrophages stimulated with chamomile 0, 10, 20, and 40 μg/mL for 16h without H2O2 and chamomile for 16h with H2O2 for 6hrs as indicated. (C) EMSA assay. EMSA was performed to determine the effect of chamomile on the nuclear translocation of Nrf2 dimers and their binding to the DNA. Controls: #1 Biotin-EBNA control DNA, #2 Biotin-EBNA control DNA+EBNA extract, #3 Biotin-EBNA control DNA+EBNA extract+20-fold molar excess of unlabeled EBNA DNA. The details of the treatment have been described in the Materials and methods section.