Figure 5.

Protein expression of NF-κB in the nuclear and cytosolic fractions extracted from the prostrate of RD and HFD fed NF-κB-Luc mice. (A) Nuclear extract were prepared as described in the ‘materials and methods’ from the prostrate of mice fed with RD and HFD. The samples were subjected to SDS-PAGE gel electrophoresis. The blots were analyzed for p65/RelA; Oct-1 was used as the loading control. The densitometric quantification for p65/RelA was normalized with Oct-1. Black bars indicate RD and white bars indicate HFD. Data are a mean of ± SD and corrected for loading. The asterisk (**) indicates the significant changes (p<0.01) in HFD compared to its respective RD fed controls. (B) Cytosolic fractions prepared as described in the ‘materials and methods’ from the prostrate of mice fed with RD and HFD. The samples were subjected to SDS-PAGE gel electrophoresis. The blots were analyzed for the indicated antibodies and β-actin was used as the loading control. Densitometric quantification represents the ratio of IκBα to p-IκBα and p-IKKβ levels. Black bars indicate RD and white bars indicate HFD. Data are a mean of ± SD and corrected for loading. The asterisk (**) indicates the significant changes (p<0.01) in HFD compared to its respective RD fed controls.