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. Author manuscript; available in PMC: 2014 May 14.
Published in final edited form as: Biochem J. 2014 Feb 15;458(1):95–107. doi: 10.1042/BJ20130685

Figure 1. Inhibition of PB or CITCO-mediated CYP2B6/CYP3A4 induction in HPHs by MG-132.

Figure 1

(A) HPHs from four donors were treated with vehicle control (0.1 % DMSO), PB (1 mM) or CITCO (2 µM) in the presence and absence of proteasome inhibitor [MG-132 (MG); 1 or 10 µM]. Total RNA, extracted after 24 h treatment, was subjected to real-time PCR analysis. The results were normalized against GAPDH and are represented as the means ± S.D. Significantly different from DMSO (*), PB (#) or CITCO (+ +), P < 0.05. (B) In a separate experiment, homogenates were harvested from liver donor HPH1900 treated for 48 h with DMSO, PB (1 mM), CITCO (2 µM) in the presence and absence of MG-132 (0.1, 1 and 10 µM). CYP2B6/CYP3A4 protein levels were assessed with Western immunoblotting.