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. Author manuscript; available in PMC: 2014 May 14.
Published in final edited form as: Biochem J. 2014 Feb 15;458(1):95–107. doi: 10.1042/BJ20130685

Figure 8. MG-132 increases the ubiquitination of hCAR.

Figure 8

COS-1 cells transfected with hCAR1 expression vector and 3 × FLAG—ubiquitin or 3 × FLAG-empty vector were exposed to chemical treatments for 5 h. Cellular extracts were precipitated with anti-FLAG M2 antibody resin. The resulting total precipitated ubiquitinated protein was subjected to Western blot detection with an anti-hCAR antibody. Lane 1, precipitates from cells transfected with hCAR1 and 3 × FLAG-empty vector; lanes 2–5, precipitates from cells transfected with hCAR1 and 3 × FLAG—ubiquitin after various chemical treatments. The lower panel shows the level of 3 × FLAG—ubiquitin input. Ab, antibody; IP, immunoprecipitation; WB, Western blot; Ub, ubiquitin.