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. 2013 Dec 20;9:713. doi: 10.1002/msb.201304750

Figure 4. The ASPP‐PP1 module provides links to cell polarity and modulates YAP1 mediated transcriptional activity.

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  1. Detailed view of the PP1‐ASPP module. The PP1‐ASPP network was defined by interaction data from ASPP1/2, PP1G, and RASF7/8/9/10 purification experiments. Three different cell polarity complexes could be linked to the PP1‐ASSP network (highlighted in blue). The polarity determinant SCRIB (Scribble) was found with PP1G, the Par polarity complex component PARD3 was identified with RASF9/10 and ASPP2, and proteins linked to planar cell polarity (VANG1, PRIC1/3 and DVL1/2/3) were co‐purified with RASF9/10. Node color corresponds to the modules defined in Fig 2.
  2. PP1‐ASPP network components affect YAP1 transcriptional activity. To test whether overexpression of PP1‐ASPP network components could influence TEAD promoter activity, HEK293‐Flp cells expressing SH‐YAP1 were co‐transfected with a firefly luciferase reporter construct containing four TEAD transcription factor binding sites together with constructs for the overexpression of indicated PP1‐ASPP network components (left panel). The measured firefly activities were normalized to the activity of a constitutively co‐expressing Renilla luciferase and a vector control. Error bars indicate standard deviation from biological triplicates. Asterisks indicate t‐test statistical significance (*P < 0.05; **P < 0.01).
  3. PP1A mediated activation of the TEAD promoter activity is dependent on YAP1. Dual luciferase assays have been performed following co‐transfection of indicated siRNA and PP1A expression construct. Error bars represent standard deviation from biological triplicates. Asterisks indicate t‐test statistical significance (*P < 0.05; **P < 0.01).