Figure 2. Both the N-terminal cysteine residues of Erv1 are required for cell viability.

Yeast complementation assays with Erv1^SXXC and Erv1^CXXS individually or co-expression via plasmid shuffling in the presence of 5-fluoro-orotic acid (FOA). Strains containing empty vector or WT Erv1 were used as negative and positive controls respectively. Cells were grown at 30°C for 3 days. OD, attenuance.