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. 2014 May 13;460(Pt 2):199–210. doi: 10.1042/BJ20131540

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(a) Time-course analysis of the redox state change of 50 μM Mia40 with the CPC motif disulfide reduced in the absence or presence of a total of 2 μM of WT or cysteine mutant Erv1 either individually or in a mixture (SXXC+CXXS) at 25°C. Reactions at designated time points were quenched by 2.5 mM AMS. (b) Oxygen consumption profiles of oxidation of the CPC motif of reduced Mia40 (50 μM) in the presence of 2 μM of WT or single cysteine mutant Erv1 as described above.