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. 2014 Apr 21;111(18):6624–6629. doi: 10.1073/pnas.1312918111

Fig. 4.

Fig. 4.

“SHAPE” probing of the env87SAM-I/IV RNA. (A) Normalized chromatogram of chemical probing of the env87(minimal) construct showing the RNA probed with N-methylisotoic anhydride in the presence and absence of SAM at 50 °C. Nucleotides showing significant changes in their reactivity are highlighted (raw gel in Fig. S4A). (B) Quantification of the reactivity differences observed in the gel shown in A. Bars above zero correspond to reactivity enhancements, whereas those below zero correspond to SAM-dependent protections of the RNA.