Table 1.

Affinities of SAM for wild-type and mutant env87 SAM-I/IV aptamers

RNA*	KD, µM	N
env87 (wild-type)	0.091 ± 0.021	1.1 ± 0.1
env87 (minimal)	0.14 ± 0.02	0.85 ± 0.03
env87 (∆U92)	0.41 ± 0.05	1.1 ± 0.1
env87 (∆U92 G93)	7.3 ± 0.4	1.0 ± 0.1
env87 (∆U92, G93, U94)	100 ± 10	0.72 ± 0.04
hybrid 1	0.27 ± 0.03	0.81 ± 0.02
hybrid 2	0.08 ± 0.01	0.84 ± 0.03
env87 (∆P4)	5.3 ± 0.5	1.10 ± 0.02

^*Binding buffer was 10 mM Na-Hepes at pH 8.0, 135 mM KCl, 15 mM NaCl, and 10 mM MgCl₂.