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Inhibition of ROS production by Nox4-shRNAs. U87MG and U251 cells transfected with Nox4-shRNA or scrambled shRNA were labeled with DCFH-DA and alterations in the intracellular ROS level were measured by FACS analysis. DCF fluorescence shown in histogram was normalized to that in scrambled cells. The data are means ± SD. *P < 0.05, versus scrambled.
