Table 5.
Effects of the A. blazei extract treatment on the respiration of brain mitochondria of old rats. The oxygen consumption, ADP/O ratio, and respiration coefficient (RC) were assessed in the mitochondria as described in Materials and Methods. The values represent the mean ± standard errors of the mean.
| Substrate | Experimental groups |
Rate of oxygen uptake (nmol min−1 mg protein−1) |
ADP/O | RC | |
|---|---|---|---|---|---|
| −ADP | +ADP | ||||
| Succinate (10 mM) | (1) Young rats | 16.11 ± 1.27 (n = 6) | 51.65 ± 2.06 (n = 6) | 1.54 ± 0.02 (n = 6) | 2.67 ± 0.14 (n = 6) |
| (2) Old rats | 14.16 ± 1.477 (n = 6) | 41.6 ± 2.68* (n = 6) | 1.22 ± 0.14 (n = 5) | 2.30 ± 0.32 (n = 6) | |
| (3) A. blazei-treated old rats | 16.01 ± 0.976 (n = 7) | 52.20 ± 3.50 (n = 6) | 1.24 ± 0.16 (n = 6) | 2.54 ± 0.27 (n = 6) | |
|
| |||||
| Pyruvate + L-malate (10 mM + 1 mM) |
(1) Young rats | 9.95 ± 0.26 (n = 6) | 40.47 ± 2.44 (n = 6) | 1.78 ± 0.10 (n = 6) | 1.92 ± 0.10 (n = 6) |
| (2) Old rats | 9.64 ± 0.71 (n = 5) | 30.71 ± 3.03# (n = 5) | 1.66 ± 0.28 (n = 5) | 1.85 ± 0.16 (n = 5) | |
| (3) A. blazei-treated old rats | 10.64 ± 0.54 (n = 7) | 35.48 ± 2.12 (n = 7) | 1.67 ± 0.04 (n = 6) | 1.95 ± 0.18 (n = 7) | |
|
| |||||
| α-Keto-glutarate (10 mM) | (1) Young rats | 4.85 ± 0.46 (n = 6) | 18.50 ± 1.39 (n = 6) | 1.88 ± 0.08 (n = 6) | 2.63 ± 0.39 (n = 6) |
| (2) Old rats | 2.99 ± 0.62# (n = 3) | 15.53 ± 1.53 (n = 3) | 2.17 ± 0.21 (n = 3) | 3.22 ± 0.90 (n = 3) | |
| (3) A. blazei-treated old rats | 3.51 ± 0.15 (n = 3) | 16.39 ± 0.81 (n = 3) | 2.60 ± 0.12** (n = 3) | 4.43 ± 2.29 (n = 3) | |
*P < 0.05 for (2) versus (1) and (2) versus (3) (ANOVA and Student-Newman-Keuls post hoc testing).
# P < 0.05 for (2) versus (1) (ANOVA and Student-Newman-Keuls post hoc testing).
**P < 0.05 for (3) versus (1) (ANOVA and Student-Newman-Keuls post hoc testing).