Figure 3.

Protective effects of SAL on H₂O₂-induced impairment of endothelium-dependent relaxation. (a) Rat thoracic aorta was pretreated with or without SAL for 30 min, and then H₂O₂ (100 μM) was added to incubate for another 30 min. After precontracting with 1 μM PE, ACh was added accumulatively. Complete relaxation of aorta induced by ACh was considered as 100%. *P < 0.05, **P < 0.01 versus control; ^† P < 0.05, ^†† P < 0.01 versus H₂O₂, n = 8–16. (b) HUVECs were pretreated with or without 10 μM SAL for 20 h and then loaded with DHE dye. After acquisition of basal data, H₂O₂ (100 μM) was added and the fluorescence was measured every 5 min within 30 min. The fluorescence of basal data was assigned the value of 100%. *P < 0.05, **P < 0.01 versus control; ^† P < 0.05 versus H₂O₂, n = 4. (c) HUVECs were pretreated with or without SAL for 20 h and then loaded with DAF-FM-DA dye. After acquisition of basal data, H₂O₂ (100 μM) was added and the fluorescence was measured every 5 min within 30 min. The fluorescence of basal data was assigned the value of 100%. *P < 0.05 versus control; ^† P < 0.05 versus H₂O₂, n = 4.