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The effects of rbr-2 and spr-5 mutations on H3K4 methylation and on the expression of selected genes in a daf-2(e1370) background. (A) A western blot of protein extracts probed with antibodies against H3K4me3, H3K4me2, and H3. (B) Relative gene expression by semi-quantitative RT–PCR in the wild-type, daf-2(e1370) and daf-2(e1370);rbr-2 and spr-5; daf-2(e1370) double mutants.
