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. 2014 Jan 14;17(2):119–125. doi: 10.1038/pcan.2013.51

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(a) Quantitative polymerase chain reaction (Q-PCR) analysis of guanyl nucleotide-releasing protein for Ras 3 (RasGRP3) in prostate cancer (PCa) cell lines represents the mean±s.d.; Q-PCR analysis of RasGRP3 mRNA was normalized to 18S. (b) Western blot analysis of RasGRP3 in PCa cell lines. (c) Downregulation of the RasGRP3 protein in PC-3 cells treated with small interfering RNA (siRNA). (d) Proliferation of PC-3 cells was inhibited after downregulation of the RasGRP3. (e) Comparisons of PC-3 migration and invasion abilities after RasGRP3 siRNA transfection. **P<0.01, ***P<0.001.