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Immunohistochemical assessments of the protein level of type II collagen. Positive staining of type II collagen declined in the nucleus pulposus at all time points after the surgery. Ligustrazine treatment completely reversed the decreased trend of type II collagen at the nucleus pulposus, while meloxicam has no obvious increasing effect. The arrow indicates the positive staining cells; the arrow head indicates the negative staining cells. Bar equals 100 μm.
