Table 1.
Sequences of primers used in the real-time RT-PCR.
| Genes | Forward primer | Reverse primer |
|---|---|---|
| Col2α1 (112 bp) | F: 5′-TCCTAAGGGTGCCAATGGTGA-3′ | R: 5′-GGACCAACTTTGCCTTGAGGAC-3′ |
| MMP 13 (142 bp) | 5′-CCCTGGAGCCCTGATGTTT-3′ | 5′-CTCTGGTGTTTTGGGGTGCT-3′ |
| Col10α1 (143 bp) | 5′-TTCACAAAGAGCGGACAGAGA-3′ | 5′-TCAAATGGGATGGGAGCA-3′ |
| β-actin (150 bp) | 5′-GGAGATTACTGCCCTGGCTCCTA-3′ | 5′-GACTCATCGTACTCCTGCTTGCTG-3′ |
| iNOS (101 bp) | 5′-CTCACTGTGGCTGTGGTCACCTA-3′ | 5′-GGGTCTTCGGGCTTCAGGTTA-3′ |
| IL-1β (120 bp) | F: 5′-AGGTCGTCATC ATCCCACGAG-3′ |
R: 5′-GCTGTGGCAG CTACCTATGTCTTG-3′ |
| COX-2 (145 bp) | F: 5′-GGAGCATCCTGAGTGGGATGA-3′ | R: 5′-AAGCAGGTCTGGGTCGAACTTG-3′ |
| MMP3 (104 bp) | F: 5′-TGGACCAGGG ACCAATGGA-3′ |
R: 5′-GGCCAAGTTCA TGAGCAGCA-3′ |
MMP-13: matrix metalloproteinase 13; IL-1β: interleukin 1β; COX-2: cyclooxygenase 2; MMP-3: matrix metalloproteinase 3; iNOS: inducible nitric oxide synthase 2.