Fig. 2.

Mito-Q₁₀ suppressed MPP⁺-induced loss of mitochondrial membrane potential. (A) N27 cells were incubated with MPP⁺ in the presence and absence of Mito-Q₁₀ for 48 h. Cells were treated for 30 min with JC-1 (3 μl/ml) and washed twice with PBS, and the fluorescence images obtained under FITC and rhodamine filter settings were overlaid. (B) The number of cells demonstrating aggregated versus monomeric JC-1 probe was quantified by measuring the ratio between the fluorescence emission at 590 (orange) and 530 nm (green) in a Nikon fluorescence microscope using Metamorph software, and the values obtained under control conditions were normalized to 1. Data shown are the means±SD of three separate experiments. *P<0.05 and **P<0.01 compared to controls; #P<0.05 compared to MPP⁺-treated group.