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. Author manuscript; available in PMC: 2014 May 14.
Published in final edited form as: Arthritis Rheum. 2002 Jan;46(1):175–190. doi: 10.1002/1529-0131(200201)46:1<175::AID-ART10015>3.0.CO;2-H

Figure 8.

Figure 8

A, Effect of CD3/CD28 stimulation and oligomycin treatment on intracellular ATP content and the ΔΨm (DiOC6 fluorescence) in normal PBLs. PBLs from 4 healthy donors were pretreated with CD3/CD28 antibodies or 2.5 μM oligomycin. Values are the mean ± SEM percentage of control cells incubated in parallel (n = 4 independent experiments). B, Effect of pretreatment with CD3/CD28 (1 hour) or oligomycin (2.5 μM for 30 minutes) on H2O2-induced elevation of the ΔΨm in PBLs from 4 healthy donors. Values are the percentage elevation of the ΔΨm elicited by exposure to H2O2 (50 μM for 20 minutes) in CD3/CD28- or oligomycin-pretreated cells compared with control cells. C, Effect of pretreatment with CD3/CD28 (1 hour) or oligomycin (2.5 μM for 30 minutes) on H2O2-induced necrosis of PBLs from healthy donors. Untreated (control), oligomycin-pretreated, and CD3/CD28-pretreated PBLs were exposed to 50 μM H2O2 for 16 hours, and necrosis was assessed by the ratio of propidium iodide–positive/annexin V–positive cells. Values are the mean ± SEM of 6 independent experiments. See Figure 1 for definitions.