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. Author manuscript; available in PMC: 2014 May 14.
Published in final edited form as: J Immunol. 1999 Feb 1;162(3):1466–1479.

FIGURE 8.

FIGURE 8

A, Cleavage of PARP (116 kDa) to 85-kDa fragment during Fas-induced apoptosis of Jurkat cells stably transfected with TAL-H expression vectors. Six hours after stimulation with 50 ng/ml Fas Ab, PARP cleavage was accelerated in L26-3/4 and L26-3/2D1 cells and was abrogated in L18-3/1 and L18-3/1D9 cells compared with that in control Jurkat cells (left panel). PARP was not cleaved in lysates of cells unstimulated with Fas Ab (right panel). Cell lysates containing 40 μg of total protein/lane were prepared and analyzed by Western blot using anti-PARP mAb C-2-10. B, Monitoring of TAL expression using Ab 170. Levels of TAL expression were reduced in L18-3/1D9 (−29%) and L18-3/1 (−48%) cells and were increased in L26-3/4 (2.5-fold) and L26-3/2D1 (+31%) cells compared with those in control Jurkat cells as previously described (27). C, Actin was detected with mAb C4.