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. Author manuscript; available in PMC: 2014 May 14.
Published in final edited form as: J Immunol. 2002 Jul 15;169(2):1092–1101. doi: 10.4049/jimmunol.169.2.1092

FIGURE 6.

FIGURE 6

Effect of IL-10 (10 ng/ml), anti-IL-10 (25 μg/ml), and IL-12 (10 ng/ml) on CD3/CD28 induced changes in Δψm (DiOC6 and JC-1 fluorescence, A), ROI production (DHR and HE fluorescence, A), and pHi (B). PBL from 10 patients with SLE were pretreated for 16 h with IL-10 (10 ng/ml), anti-IL-10 (25 μg/ml Ab), or IL-12 (10 ng/ml), then washed and stimulated with CD3 and CD28 mAbs. The Δψm and pHi were assessed 1 h, while ROI production was determined 6 h after CD3/CD28 costimulation. Values of p reflect significant enhancement of CD3/CD28-induced ROI production and elevation of pHi following pretreatment with anti-IL-10 and IL-12.