Table 3.

Commercially available immunoassays for ANA testing.

Antigen	Automated screening assays					Multiplex assays			Line immunoassays/dot blots
	EliA CTD	EliA Symphony	QUANTA Flash ENA7	QUANTA Flash CTD Screen Plus	Alegria ANA Detect	Bio-Plex 2200 ANA ALBIA	AtheNA Multi-Lyte Anti-Nuclear Antibodies (ANA)	FIDIS Connective 10	ANA Profile	ImmcoStripe ANA Advanced	ANA12 IgG BlueDot	ANA-12 Pro (BLOT)	recomLine ANA/ENA IgG	ANA Lia MAX
	Thermo Scientific	Thermo Scientific	INOVA Diagnostics	INOVA Diagnostics	Orgentec	Bio-Rad	Zeus	Theradiag	Euroimmun	IMMCO Diagnostics	D-Tek	AESKU Diagnostics	MIKROGEN Diagnostics	HUMAN Diagnostics
U1-RNP	X	X	X	X		X	X		X		X	X	X
U1-RNP/Sm		X	X	X	X			X			X
U1-RNP 68 kDa					X					X				X
U1-RNP A					X					X				X
U1-RNP C					X					X				X
SS-A/Ro60	X	X	X	X	X	X(i)	X	X	X	X	X	X	X	X
Ro52/TRIM21	X	X	X	X	X	X(i)			X	X		X	X	X
SS-B/La	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Centromere	X	X		X#	X	X	X	X		X	X#	X	X	X
Scl-70/topo I	X	X	X	X	X	X	X	X	X		X	X	X	X
Jo-1	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Fibrillarin/U3RNP	X
RNA-Pol III	X			X
Ribosomal P	X			X		X		X	X	X	X	X	X	X
PM/Scl	X			X					X					X
PM/Scl-75										X
PM/Scl-100					X					X
PCNA	X			X					X	X	X		X	X
Mi-2	X			X					X	X				X
Sm	X			X	X	X	X		X	X	X	X	X	X
dsDNA	X			X	X	X		X	X	X		X	X	X
Nucleosome					X				X	X		X		X
Histone					X		X		X	X		X	X	X
Ku				X					X	X	X			X
SRP54										X
AMA-M2									X	X				X

Note: some companies offer several line immunoassays for ANA detection. Most comprehensive assays from different companies are shown.

AMA: Anti-mitochondrial antibodies; PCNA: Proliferating cell nuclear antigen; SRP: signal recognition particle.

NOTE: RNP and RNP/Sm contain the subunits RNP-A, RNP-C and RNP-68 kDa.

⁽ⁱ⁾Reported outside the United States as Ro60 and Ro52 and as SS-A in the United States.

^#Contains CENP-A and CENP-B.