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. 2014 Apr 28;2014:696218. doi: 10.1155/2014/696218

Figure 1.

Figure 1

Effects of different durations of hypoxia/reoxygenation on BV2 microglia cells. (a) BV2 cells were exposed to hypoxia for 6 or 12 hours followed by reoxygenation for 12 hours. Morphological changes were observed under an inverted microscope (×100). (b) After exposure to hypoxia for 12 hours, the BV2 cells were reoxygenated for 12 or 24 hours. Cell viability was assed using the MTT method. Each value indicates the mean ± SD. N = 5.  ## P < 0.01 compared to the control group. (c) ROS level in BV2 microglial cells after hypoxia for 12 hours and reoxygenation for 12 hours based on flow cytometry. N = 4.  ## P < 0.01 compared to the control group.