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. 2014 Mar 7;33(5):1122–1130. doi: 10.3892/ijmm.2014.1690

Figure 1.

Figure 1

Mouse microglia express both Plexin-A1 and Neuropilin-1. (A) RT-PCR detects mRNAs of Plexin-A1, Neuropilin-1, and Sema3A in mouse hippocampus, and Plexin-A1 and Neuropilin-1 in primary microglia. The method detects no Plexin-A1 mRNA in Plexin-A1-deficient (−/−) hippocampus or Plexin-A1−/− primary microglia. (B) Western blotting detects Plexin-A1, Neuropilin-1, and Sema3A protein in mouse hippocampus, and Plexin-A1 and Neuropilin-1 protein in primary microglia. Immunoblotting detects no Plexin-A1 protein in the Plexin-A1−/− hippocampus or Plexin-A1−/− primary microglia. WT, wild-type; A1−/−, Plexin-A1−/− hippocampus or Plexin-A1−/− primary microglia; Nrp1, Neuropilin-1; RT-PCR, reverse transcriptase polymerase chain reaction.