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. 2014 Mar 14;9(5):1575–1582. doi: 10.3892/mmr.2014.2053

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Copyright © 2014, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Characterization of rat BMSCs. (A) Spindle-shaped cells formed a colony in primary cells and cells after passaging several times (P5) remained with their fibroblast-like morphology (magnification, ×40); (B) Differentiation potentials of BMSCs: osteogenic differentiation (Oil Red O staining; magnification, ×100), adipogenic differentiation (Alizarin Red S staining; magnification, ×200) and chondrogenic differentiation (Toluidine Blue staining; magnification, ×100). (C) Several markers of BMSCs identified by flow cytometry. BMSCs, bone marrow mesenchymal stem cells.