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qRT-PCR of the mRNA expression of PI3K, Akt, PTEN and RhoA at the implantation site in the inhibitor group injected with LY294002 (L) and the control group (Con; not injected). β-actin was used as the internal control. The experiment was performed 3 times. Three mice were used in each experiment. *P<0.05 and **P<0.01 indicates statistical significance between the pregnant group and the inhibitor group.
