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. 2014 Mar 7;9(5):1634–1640. doi: 10.3892/mmr.2014.2028

Figure 4.

Figure 4

Involvement of the ERK1/2 and JAK2/STAT3 pathways in retinol binding protein 4 (RBP4)-mediated enhancement of rat aortic smooth muscle cell (RASMC) proliferation. (A) Proliferation of RASMCs measured by the MTT assay. RBP4-induced proliferation of RASMCs is inhibited in the presence of the JAK1/2 inhibitor PD98059 (5×10−5 M) but not of the JAK2 inhibitor AG490 (5×10−5 M). Data come from 3–8 independent experiments. *P<0.05, **P<0.01 vs. Con; ##P<0.01 vs. Ins (10−5 M) + RBP4 (4 μg/ml). (B) Cell cycle analysis by flow cytometry. Control, untreated cells; Ins + RBP4, cells treated with 10−5 M Ins + RBP4 (4 μg/ml) for 24 h; +PD98059, cells pre-treated with PD98059 (5×10−5 M) for 10 min prior to treatment with insulin and RBP4; +AG490, cells pre-treated with AG490 (5×10−5 M) for 10 min prior to treatment with insulin and RBP4; DMSO, wild-type cells treated with 0.1% DMSO (PD98059 isovolumetric).