Figure 3.

Effect of the actin cytoskeleton reorganization induced by Jas or Cyto D on the activation of HSC-T6 cells. (A) HSC-T6 cells were treated with dimethylsulfoxide, Jas or Cyto D, respectively. The gene expression of α-SMA and collagen type 1 was assessed by the quantitative polymerase chain reaction. Data were analyzed using the 2^−ΔΔCt method. (B) The protein expression of α-SMA and collagen type 1 was assessed by western blot analysis. Following treatment, cell lysates were resolved using 12% SDS-PAGE, followed by transfer to a polyvinylidene fluoride membrane. Western blot analysis was performed with specific antibodies and each band was detected using enhanced chemoluminescence reagent. Data are expressed as the mean ± standard error of five experiments. ^*P<0.05. α-SMA, α-smooth muscle actin; IOD, integrated optical density; Cyto D, cytochalasin D; Jas, jasplakinolide.