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. 2014 Mar 11;9(5):1641–1647. doi: 10.3892/mmr.2014.2036

Figure 4.

Figure 4

Actin cytoskeleton reorganization-induced HSC-T6 cell activation correlates with the p38 MAPK pathway. (A) HSC-T6 cells were pretreated with PD98059 (10 μmol/l) or SB203580 (100 nmol/l), respectively, for 30 min. The cells were then exposed to DMSO or Jas. Gene expression of α-SMA and collagen type 1 was then determined using the quantitative polymerase chain reaction. (B) The phosphorylation status of p-38 MAPK was assessed by western blot analysis. HSC-T6 cells were incubated with DMSO or Jas for 1 h. Cell lysates were resolved using 12% SDS-PAGE, followed by transfer to a polyvinylidene fluoride membrane. Western blot analysis was performed with specific antibodies to distinguish between different phosphorylation statuses of p38 MAPK. In addition, total p38 MAPK was analyzed as a loading control. P-p38 MAPK was densitometrically analyzed and normalized to total p38 MAPK. The results are expressed as the mean ± standard error of five experiments. *P<0.05. α-SMA, α-smooth muscle actin; IOD, integrated optical density; Jas, jasplakinolide; DMSO, dimethylsulfoxide; p38 MAPK, p38 mitogen-activated protein kinase; P-p38, phosphorylated p38 MAPK.