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. 2014 Feb 27;33(5):1083–1088. doi: 10.3892/ijmm.2014.1678

Figure 5.

Figure 5

Apoptosis in EC9706 cells treated with different drugs after 24 h. (a) The four groups (blank control group, 100 μg/ml DDP group, 5 μM MG132 group, 100 μg/ml DDP and 5 μM MG132 group) were incubated for 24 h, cells were stained with FITC-conjugated Annexin V and PI, followed by flow cytometric analysis. The percentages of apoptotic cells were obtained from three independent experiments (*P<0.05 vs. the control group, **P<0.01 vs. DDP and MG132 group). (b) Early apoptosis was defined by Annexin V+/PI- staining (B4), and late apoptosis was defined by Annexin V+/PI+ staining (B2). (c) Cells of the four groups were stained with Annexin V-FITC and PI. Images captured under a microscope; magnification, ×400.