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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Exp Neurol. 2014 Jan 15;254:78–89. doi: 10.1016/j.expneurol.2014.01.006

Fig. 4. Quantification of PAS-positive cytoplasmic inclusions and PAS-negative vacuoles in HMOX1-transfected and control rat astrocytes.

Fig. 4

CA-like structures were identified on morphological grounds (panel c) and quantified as total vacuoles (tailed arrows), vacuoles containing or surrounded by Flag-HO-1-immunoreactive material (black arrows), and PAS-positive inclusions (white arrows). Calculations were expressed as number of vacuoles per cell at day 2 (panel a) and day 3 (panel b) post-transfection. N = 4–5 glial monolayers per group from several independent cultures.